Eppendorf BioSpectrometer basic Manuel d'utilisateur télécharger pdf (Page 9)

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BioSpectrometer basic
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Operating instructions

Eppendorf BioSpectrometer

basic

English (EN)

1.4 Abbreviations used

Absorbance

DNA

Deoxyribonucleic acid

dsDNA

Double-stranded DNA

Dye methods

Methods for dye labels group for measuring dyed biomolecules

FOI

Frequency of Incorporation: measure for the amount of dye molecules in relation to the amount of

nucleotides in dyed biomolecules

mol/L (molar)

OD600

Optical density at a wavelength of 600 nm

RNA

Ribonucleic acid

ssDNA

Single-stranded DNA

Ultraviolet radiation

Vis

Visible light

Coefficient of variation (standard deviation/average value) in percent

1 2 3 4 5 6 7 8 9 10 11 12 13 14 ... 103 104

Eppendorf BioSpectrometer 1
Table of contents 3
1 Operating instructions 7
1.1 Using this manual 7
1.3 Symbols used 8
1.4 Abbreviations used 9
Operating instructions 10
English (EN) 10
2 Product description 11
2.1 Main illustration 11
2.2 Delivery package 11
2.3 Features 12
3 Safety 13
3.1 Intended use 13
3.2 User profile 13
3.3 Warnings for intended use 13
3.3.2 Damage to device 14
 Do not open the device 16
4 Installation 17
4.1 Preparing installation 17
4.2 Selecting the location 17
4.4 Connecting the printer 17
4.4.2 Thermal printer DPU-414 18
Installation 20
5 Operation 21
5.1.1 Entering text 23
5.2 Inserting the cuvette 24
5.3.2 Measuring procedure 25
5.3.2.1 Selecting a method 25
5.3.2.2 Checking parameters 26
5.3.2.4 Measuring samples 27
5.3.2.5 Finalizing the method 27
Operation 30
6 Methods 31
6.1 Selecting a method 31
6.2.1 Absorbance method group 32
6.2.2 Routine method group 33
6.2.3 Basic method group 34
6.3 Method parameters 35
6.4 Method procedure 40
6.4.1 Check parameters 41
6.4.2 Measure standards 42
6.4.3 Measure samples 43
6.4.5 Process results 50
6.4.7 Print & export 55
7 Functions 59
7.1.1 Results memory 60
 Confirm with enter 61
7.1.4 Device settings 64
7.1.5 Device calibration 64
7.1.6 Info 65
Functions 66
8 Maintenance 67
8.1 Cleaning 67
8.3 Checking the device 69
Maintenance 70
8.4 Replacing fuses 73
9 Troubleshooting 75
9.1 General errors 75
9.2 Error messages 76
9.3 Result flags 79
Troubleshooting 82
10.1 Transport 83
10.2 Storage 83
10.3 Disposal 83
11 Technical data 85
11.1 Power supply 85
11.2 Ambient conditions 85
11.3 Weight/dimensions 85
11.4 Photometric properties 86
11.6 Application parameters 87
Technical data 88
12 Evaluation procedure 89
12.1 Absorbance values 89
12.1.3 Cuvette correction 90
12.4 Dilution 92
YYYXXXcorrXXX 93
ACFAA u 93
S, total 94
12.6.2 Calculation of the FOI 96
12.7 Dual wavelength 97
Evaluation procedure 98
13 Ordering information 99
Ordering information 100
Evaluate your manual 104

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Eppendorf BioSpectrometer basic Manuel d'utilisateur Page 9

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