Eppendorf AF2200 Plate Reader Manuel d'utilisateur

nualPlateReader AF2200EN)g manual Eppendorf® PlateReader AF2200Operating manual

Operating instructionsEppendorf® PlateReader AF2200English (EN)10

Product descriptionEppendorf® PlateReader AF2200English (EN)112 Product description2.1 Main illustrationAbb. 2-1: Front and rear viewFig. 2-1: Front a

Product descriptionEppendorf® PlateReader AF2200English (EN)122.3 FeaturesThe PlateReader AF2200 is well-suited for the quantification of biomolecules

Product descriptionEppendorf® PlateReader AF2200English (EN)132.4 System requirementsTo install the software, the system must meet the following minim

Product descriptionEppendorf® PlateReader AF2200English (EN)14Drive, graphic card 1xCD-ROM driveWindows Vista:DirectX 9 graphic and 32 MB graphics mem

Product descriptionEppendorf® PlateReader AF2200English (EN)152.5 Fluorescence detection systemAbb. 2-2: Upper fluorescence dete ction system (fluores

Product descriptionEppendorf® PlateReader AF2200English (EN)16Abb. 2-3: Lower fluorescence detect ion system (fluorescence bottom reading)Fig. 2-3: Lo

Product descriptionEppendorf® PlateReader AF2200English (EN)17Flash lampThe device uses a high-performance xenon gas-discharge lamp (flash lamp). The

Product descriptionEppendorf® PlateReader AF2200English (EN)18The lens systemThe lens system bundles the maximum amount of fluorescent light from a we

Product descriptionEppendorf® PlateReader AF2200English (EN)192.6 Absorbance measurement systemA light path similar to the one used for fluorescence e

6141 900.208-02/072013Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyri

Product descriptionEppendorf® PlateReader AF2200English (EN)202.6.1 Light source systemThe absorbance light source system is similar to the upper fluo

SafetyEppendorf® PlateReader AF2200English (EN)213Safety3.1 Intended useThe PlateReader AF2200 is designed for use in molecular biology, biochemistry

SafetyEppendorf® PlateReader AF2200English (EN)223.3.2 Damage to deviceWARNING! Damage to health from toxic, radioactive or aggressive chemicals as we

SafetyEppendorf® PlateReader AF2200English (EN)23NOTICE! Function impairment due to mechanical damage. After mechanical damage to the device, ensure

SafetyEppendorf® PlateReader AF2200English (EN)243.4 Information on product liabilityIn the following cases, the designated protection of the device m

InstallationEppendorf® PlateReader AF2200English (EN)254 Installation4.1 Preparing installation Use the details included in the delivery package to c

InstallationEppendorf® PlateReader AF2200English (EN)26When the device is delivered, the plate compartment is secured using a transport lock to preven

InstallationEppendorf® PlateReader AF2200English (EN)274.3 Selecting the locationFind a suitable location for the device according to the following cr

InstallationEppendorf® PlateReader AF2200English (EN)28

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)295 Inserting the filter slide5.1 Information on optical filters5.1.1 Fluorescence

Table of contentsEppendorf® PlateReader AF2200English (EN)3Table of contents1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . .

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)305.2 Filter slides and filter orientation5.2.1 Filter slidesThe filter slides cons

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)31Abb. 5-3: Filter without marking (arrow indicates the direction of the light)Fig.

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)325.3.2 Inserting user-specific filters5.3.3 Defining filtersThe device automatical

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)332. Adjust the filter definitions as follows:The Overview tab and four tabs for th

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)34Wavelength: Enter the filter wavelength in the following area.(1) Fluorescence in

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)35When a filter is replaced, this information will be lost if the last flash counte

Inserting the filter slideEppendorf® PlateReader AF2200English (EN)36

OperationEppendorf® PlateReader AF2200English (EN)376 Operation6.1 Overview of operating controls6.1.1 Notes on operationMake sure that the computer i

OperationEppendorf® PlateReader AF2200English (EN)38Before the start of each measurement, make sure that microtest plate position A1 is correctly inse

OperationEppendorf® PlateReader AF2200English (EN)396.2.4 Multi mode measurementsThe software offers multimode measurements. This means that 10 measur

Table of contentsEppendorf® PlateReader AF2200English (EN)45.3 Installing user-specific filters . . . . . . . . . . . . . . . . . . . . . . . . . . .

OperationEppendorf® PlateReader AF2200English (EN)40In this equation, U is the voltage, Gain is the selected amplification, 255 is the maximum amplifi

OperationEppendorf® PlateReader AF2200English (EN)41All standard microplates with 6 to 384 wells (maximum plate height: 23 mm incl. lid) that comply w

OperationEppendorf® PlateReader AF2200English (EN)426.4.4 MRW typeThe MRW type defines the pattern according to which the measurement is performed. Yo

OperationEppendorf® PlateReader AF2200English (EN)436.4.5 MRW sizeThe MRW size determines the number of points to be measured in a well. Depending on

OperationEppendorf® PlateReader AF2200English (EN)446.4.7 Displaying results in Microsoft ExcelA graphic, schematic overview of the measuring points i

TroubleshootingEppendorf® PlateReader AF2200English (EN)457 Troubleshooting7.1 General errorsError Possible cause RemedyMeasuring results are imprecis

TroubleshootingEppendorf® PlateReader AF2200English (EN)467.2 Error messagesIf a system error occurs, the error message appears on the screen of the c

MaintenanceEppendorf® PlateReader AF2200English (EN)478 Maintenance8.1 Decontamination before shipmentIf you are shipping the device to the authorized

MaintenanceEppendorf® PlateReader AF2200English (EN)488.3 Replacing air filters8.4 Cleaning1. Wipe the surfaces with a cloth that has been dampened wi

MaintenanceEppendorf® PlateReader AF2200English (EN)498.5 Disinfection/Decontamination1. Remove the microplate from the device.2. Switch off the devic

Table of contentsEppendorf® PlateReader AF2200English (EN)59 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

MaintenanceEppendorf® PlateReader AF2200English (EN)508.6.1 Test criteriaAll specifications can be changed without prior notification.Microplate speci

MaintenanceEppendorf® PlateReader AF2200English (EN)518.6.2 Test instructions, upper fluorescenceThe following tests can be conducted on the device to

MaintenanceEppendorf® PlateReader AF2200English (EN)52Material/reagents• 1 nM fluorescein (in 0.01 M NaOH) (fluorescein sodium salt)• 0.01 M NaOH (=bl

MaintenanceEppendorf® PlateReader AF2200English (EN)53Pipette 200 µL of 1 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding we

MaintenanceEppendorf® PlateReader AF2200English (EN)54Precision from one measurement to the nextCarry out the following measurement to determine the p

MaintenanceEppendorf® PlateReader AF2200English (EN)55Calculating the precision from one measurement to the nextThe result of the formula specifies th

MaintenanceEppendorf® PlateReader AF2200English (EN)56Pipette 200 µL of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding w

MaintenanceEppendorf® PlateReader AF2200English (EN)57Precision across the plateCarry out the following measurement to determine the precision across

MaintenanceEppendorf® PlateReader AF2200English (EN)58Calculating the precision across the plateThe results of the formula specifies the precision acr

MaintenanceEppendorf® PlateReader AF2200English (EN)59Pipette 200 µL of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the corresponding w

Table of contentsEppendorf® PlateReader AF2200English (EN)6

MaintenanceEppendorf® PlateReader AF2200English (EN)608.6.4 Absorbance test instructions - baseline stabilityConduct the following measurements to det

Technical dataEppendorf® PlateReader AF2200English (EN)619 Technical data9.1 Power supply9.2 Weight/dimensions9.3 Ambient conditions9.4 Measuring meth

Technical dataEppendorf® PlateReader AF2200English (EN)629.5 Specifications 9.6 Fluorescence intensityUse Universal lab deviceInterface USBMeasurement

Technical dataEppendorf® PlateReader AF2200English (EN)639.6.1 Definition of the detection limitThe detection limit is the fluorophore concentration a

Technical dataEppendorf® PlateReader AF2200English (EN)649.7 AbsorbanceThe following specifications apply for a • wavelength range of 300 – 700 nm and

Ordering InformationEppendorf® PlateReader AF2200English (EN)6510 Ordering InformationOrder no. (International)Order no. (North America)Description61

Ordering InformationEppendorf® PlateReader AF2200English (EN)660030 621.670 951040503Microplate 384/V-PP80 plates, wells white, gray border colorPCR c

Transport, storage and disposalEppendorf® PlateReader AF2200English (EN)6711 Transport, storage and disposal11.1 Transport Use the original packaging

Transport, storage and disposalEppendorf® PlateReader AF2200English (EN)6811.3 Storage11.4 DisposalIn case the product is to be disposed of, the relev

EG-Konformitätserklärung EC Conformity Declaration Das bezeichnete Produkt entspricht den einschlägigen grundlegenden Anf

Operating instructionsEppendorf® PlateReader AF2200English (EN)71 Operating instructions1.1 Using this manual Read this operating manual completely b

Evaluate your manualGive us your feedback.www.eppendorf.com/manualfeedbackYour local distributor: www.eppendorf.com/contactEppendorf AG · 22331 Hambur

Operating instructionsEppendorf® PlateReader AF2200English (EN)81.3 Symbols usedDepiction Meaning 1.2.Actions in the specified order Actions withou

Operating instructionsEppendorf® PlateReader AF2200English (EN)91.4 Abbreviations usedAAbsorbanceANSIAmerican National Standards InstituteDNADeoxyribo