Eppendorf UVette Manuel d'utilisateur télécharger pdf (Page 6)

Eppendorf® UVette® — Instructions for use

4.1.1 Eppendorf BioPhotometer/Eppendorf BioSpectrometer

1. Position the cuvette so the required optical path length points toward the light path.

2. During insertion, press the cuvette all the way down against a light resistance.

4.1.2 Inserting the cuvette and adapter in photometers from other

manufacturers

Function of the adapter

• Adjust the height of the light beam in the corresponding photometer.

• Diaphragm function:

– Hides the light bundle on the cross-section of the UVette measuring window

– Reduction of stray light

Like measurements with commercial, quartz glass ultra-micro cuvettes, hiding the light

bundle reduces the upper limit of the photometric measuring range. Therefore, the

usable, linear measuring range may be lower than the value specified for the

photometer. To calculate the highest absorbance values that can be measured with the

adapter/UVette, determine the measuring range by measuring a dilution series of the

desired wavelength.

Additional information is available from Application Support (contact information is on

the last page of these instructions) or at: www.eppendorf.com.

1. Select the adapter that matches the light path height of the device.

2. Insert the UVette in the adapter.

3. Insert the adapter and UVette in the cuvette holder of the photometer. Firmly press

the UVette downward.

The UVette and adapter may not have any lateral play in the cuvette shaft of the

device.

4.2 Measuring blanks and samples

Hint!

Precise and reproducible positioning of the adapter and cuvette in the cuvette

holder is a decisive factor for the reproducibility of the measurement.

NOTICE!

NOTICE! Faulty measured values due to incorrect orientation of the

cuvette.

 Always insert the cuvette in the same direction within a series of

measurements. Do not rotate the cuvette 180° during a series of

measurements.

NOTICE!

NOTICE! Faulty measured values due to repeated use of the cuvette.

If the UVette is used several times, material changes can lead to incorrect

measured values.

 Each UVette may only be used for a single measuring procedure with one

blank value and one sample value.

 The cuvette blank must be determined again for each cuvette.

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Eppendorf UVette Manuel d'utilisateur Page 6